Breadcrumb
Neurotrophins BDNF and NT4/5 accelerate dental pulp stem cell migration
What is it?
This study focused on the expression of particular NTs and how they could promote the migration of DPSCs to where they can regenerate tissue.
What problem does it aim to solve?
Promoting tissue regeneration is an important part of dental treatment. Human Dental Pulp Stem Cells (DPSCs) are an important part of tissue regeneration — they can grow into different kinds of tissue and regenerate themselves. Neurotrophins (NTs) are growth factors that can feed and sustain neurons and promote their survival and growth. They could play an important role in potential treatments.
How does it work?
Human DPSCs were analyzed in the laboratory, ultimately finding that neurotrophin family members BDNF and NT4/5 accelerated DPSCs migration in vitro.
What are the real-world implications?
Future novel drug candidates could emerge.
What are the next steps?
Future work could focus on how different kinds of stimuli impact expression of different neurotrophic factors, as well as how various neurotrophic factors affect DPSCs.
Expression of neurotrophic factors and cell adhesion molecules in human DPSCs (A) Expression of the neurotrophic factors Ngf, Bdnf, Nt3, Nt4/5, their receptors TrkB, TrkC, and the co---receptor p75 in the human DPSCs. 10% samples were loaded for internal control actin (B) Cellular localization of BDNF, its receptor TrkB, and co---receptor p75 in DPSCs (C) ELISA of the secreted BDNF from DPSCs in the culture medium (n = 5) (D) immunofluorescent staining and (E) Western blotting of integrin a5, integrin b1 and laminin in the human DPSCs.
Source
Neurotrophins BDNF and NT4/5 accelerate dental pulp stem cell migration, Biomedical Journal, April 2020
Authors
Nan Xiao, Der Thor, Wei Ye Yu, Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific
